Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction.
AIM To establish a competitive PCR (cPCR) assay for quantitation of H pylori organisms in dental plaque samples. METHODS The cPCR co-amplified target H pylori DNA and a known amount of internal standard template in the same tube with the same primers directed to 0.86 kb DNA of H pylori. The internal standard was a synthesised DNA bearing the same primer recognition sites at two ends and a non...
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A 1.9-kb cloned fragment of chromosomal DNA randomly selected from a Helicobacter pylori cloned library was evaluated as a potential probe. The probe detected 19 of 19 H. pylori strains and yielded a specificity of 98.7% when tested against 306 other bacterial strains representing 32 different species. False-positive results with non-H. pylori strains were due to the presence of contaminating v...
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The aims of this study were to detect Helicobacter pylori (H. pylori) by culture, polymerase chain reaction (PCR) and histopathological methods, to determine the prevalence of active H. pylori infection in Elazig Province, East of Turkey and to evaluate the relationship between H. pylori infection and sex. Antrum and corpus samples of 184 Turkish patients (85 male and 99 female, age range 17 to...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.4.783-787.1993